公山羊Protamine 1 mRNA表达特性及与精液品质相关性的研究

doi:10.11843/j.issn.0366-6964.2016.07.007

摘要/Abstract

摘要：

旨在研究雄性山羊主要繁殖器官中PRM1 mRNA表达特性，分析精子PRM1 mRNA表达与精液品质参数的相关性。本研究通过荧光定量PCR技术分析PRM1 mRNA表达规律，利用免疫组化技术对睾丸中PRM1蛋白进行定位，并应用GraphPad Prism 5软件分析精子PRM1 mRNA表达量与精液品质指标的相关性。结果显示，PRM1 mRNA在睾丸中高度表达，其次是附睾，睾丸中PRM1 mRNA表达量是附睾头的247倍，附睾头显著高于附睾体和尾（P<0.05），在剩余其他组织中微量表达。在周岁之前，睾丸中PRM1 mRNA表达量随着月龄增加而增加，12月龄的表达量显著高于9月龄的表达量（P<0.05），9月龄表达量显著高于其他低月龄的表达量（P<0.05）。精子PRM1 mRNA的表达量与精子活力（R2=0.586 0，P=0.000 5）、精子密度（R2=0.442 2，P=0.004 9）呈显著正相关。山羊PRM1在长形精子细胞和精子细胞中表达，睾丸其他生精细胞及间质细胞中无表达。山羊PRM1 mRNA表达具有时空表达特性，PRM1 mRNA表达量可以作为评价公羊繁殖力的指标。

Abstract:

The aims of this study were to evaluate the expression profiles of Protamine 1（PRM1） mRNA in reproductive organs of bucks and to analyze the correlation between expression of PRM1 mRNA in spermatozoa and semen quality index.In this experiment，mRNA expression was detected by real-time fluorescent quantitative PCR.Cellular localization of PRM1 in testis was examined by immunohistochemistry.The correlation was analyzed by GraphPad Prism 5 software.The results showed that：the expression of PRM1 mRNA in testis was the highest in all tissue detected，which was 247 times higher than that in the epididymal caput（P＜0.05）.The expression of PRM1 mRNA in epididymal caput was significantly higher than that in epididymal corpus and cauda（P<0.05）.The expression of PRM1 mRNA in other tissues was very few.The expression of PRM1 mRNA in testis was gradually increased with the increase of ages in one year old.The PRM1 mRNA expression in testis at 12 months old were significantly higher than that at 9 months old（P＜0.05）.The PRM1 mRNA expression in testis at 9 months old was significantly higher than that in other ages.Concentration of PRM1 mRNA in sperm was positively correlated with the sperm motility （R2=0.586 0，P=0.000 5） and sperm concentration （R2=0.442 2，P=0.004 9）.PRM1 was located in elongating spermatids and spermatozoa in testis of bucks.There was no positive signal in other cells of testis.This study indicated that the expression of PRM1 mRNA was spatial and temporal-specific pattern.The sperm PRM1 mRNA content could be index of fertility of bucks.

中图分类号:

S827.2

张春香，赵辉，郭丽娜，郑亚琳，张彩霞，刘文忠，乔利英，任有蛇. 公山羊Protamine 1 mRNA表达特性及与精液品质相关性的研究[J]. 畜牧兽医学报, 2016, 47(7): 1354-1362.

ZHANG Chun-xiang，ZHAO Hui，GUO Li-na，ZHENG Ya-lin，ZHANG Cai-xia，LIU Wen-zhong，QIAO Li-ying，REN You-she. Expression Profiles of Protamine 1 mRNA and Correlation with Index of Semen Quality in Bucks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1354-1362.

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